The effect of q-RT-PCR analysis method on saline gargle samples in SARS-CoV-2 clinical diagnostic methods

COVID‑19 is a devastating disease, and its control difficult due to high transmissibility rate long incubation average period (6.4 days). Additionally, more than half of the infected patients were asymptomatic young people or children. The virus transmission actual challenge controlling disease. Because limited treatment options, diagnosis techniques have been first focus all over world, involving q-RT-PCR as gold standard, serological tests, point care studies, RT-LAMP. Generally, nasopharyngeal, oropharyngeal samples are preferred clinically sources. However, alternative sources being researched, particularly for healthcare professionals who difficulty taking samples, afraid giving pediatric patients. Herein, physiological saline has utilized offer an source besides swab use in q-RT-PCR. In this study, 212 randomly chosen patients’ studied, we evaluated concordance accurate results two different sources, obtained from gargle utilized, which widely used medically recommended irrigating wound dressing solution. We our experiments with method, confidence interval determines 74.50% positivity when compared routine procedure summarized. addition, only sampling method studied low-income countries, cost testing COVID-19 will decrease significantly. does not require vNAT VTM transport solution sterile sticks shown. plastic container lid patient can SF spit it out ideal this. provides great cost-benefit countries.